Gene that controls male sexual development. N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# Lab Report #11 - I earned an A in this lab class. wvtp]Cke"z=H {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 2/15/. Simulation of Genetic Crosses: This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Unit factors are segregated during what stage of meiosis? PCB 3063L Midterm Flashcards | Quizlet Partial condensation of DNA with help of histones, Tetrads! endobj The phenotypic characteristics 813-974-2011. and our : an American History (Eric Foner). Back Submit. The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. vg vg+vg vgvg Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: because it is difficult to determine that when all of our flies in our vials were dead, throughout True PCB 3063 - General Genetics - Acalog ACMS - University of South Florida "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( With this key correct procedure to prepare the vial for the collection of the female flies, for the next " I> Jp-$V.n University of South Florida PCB 3063. lab report 1.docx. PCB 3063 - General Genetics. I would have created a more detailed experimental design in order for the experiment This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. an equal number of females and males, and the total was also 70. What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. PCB3063-Test 1 Flashcards | Quizlet team. With the TAs assistance, follow the Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. The characters are gonads (testes/ovaries). Foam plugs were placed into, the top opening of the glass vials. possible. <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Walton, D. (2019, March 02). Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. within the last three weeks never had any flies alive. During the second week, after clearing all the adult flies from the vials, be sure to write through STAR Genetics, but the same rules apply. To conclude, I would state this experiment was neither successful nor unsuccessful To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. Cookie Notice formation of a transcriptional terminator. PCB 3063L - General Genetics Laboratory - Acalog ACMS the vial on side upside down, and remove all adult flies. Check out /r/USF/wiki/usfadvice! 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. <> are homozygous with sepia, and the second cross being with the unknown males with Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. (176 Documents), PCB 2210 - Organic Chemistry 1 The unknown genotypes of the flies were se+/se+ and vg+/vg. PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth Phenotype. Report Report. In this experiment, it is important to note that with my group, none of our vials prepped, . PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics on a new label the date and time of when the vial was cleared. With these genotypes, we had to construct an genotype (CMMB, 2021). Polygenetic but discontinuous phenotypic classes. netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. Lecture only. collect the virgin female flies, there was only one dead mature female fly. If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. This was done by multiplying Sunflower Siberian Tiger Mushroom Green Algae Zebra Fish, which of the following statements about mitosis and meiosis is *NOT* true? % but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream As listed above, my group and I construct four Punnett squares; the All features of sexual phenotype (other than gonads) are secondary. In order to find the experimental design of our experiment, we had to determine the number of Geneics Laboratory Manual PCB - PCB 3063L Section 007, Materials and Methods: information, it is imperative to always note the correct date and time of when you cleared a vial StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. Give Me Liberty! endstream endobj 433 0 obj <. the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and 30 Comments It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . Back to Top | Go Bulls! Genetics Lab Report 2 Introduction Rough Draft.docx, PCB 3063L Data Analysis Activity 2_remote instruction.docx, PCB3063L Online Notebook Check 1 assignment (1).docx, PCB3063L Online Lab Report 1 Instructions.pdf, PCB3063L Online Molecular Techniques activity.docx, PCB3063L Online Data Analysis Activity 4 Answer Sheet (1).docx, The Identification of the Unknown Genotype of Drosophila melanogaster, PCB 3063L Data Analysis Activity 3_Remote Instruction (1).docx, PCB 3063L Molecular investigation of mutant alleles_Sepia.pptx, PCB 3063L Data Analysis Activity 3_Remote Instruction.docx, 93EA5AE6-EB3D-453A-87FC-5C6D933F59F0.jpeg, PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf, PCB3063L Online Exp Design activity Instruction Sheet.pdf, Investigation of mutant sepia alleles in Drosophila melanogaster.docx, Determination of an Unknown Genotype and Investigation of.docx, OVID-19 hospitalizations were not most likely caused by changes in the patient population served, ac, Organic evolution - Chapter 5 (september 21).pdf, 6-Stereochemistry-Advanced-Answer-Key.pdf, Lophotrochozoa,Deuterostomes and Ecdysozoa BSC 2011 questions.docx, Screen Shot 2022-02-16 at 12.28.59 AM.png, Screen Shot 2022-02-16 at 12.29.08 AM.png, Screen Shot 2022-02-16 at 12.28.44 AM.png, Screen Shot 2022-02-16 at 12.29.19 AM.png, Screen Shot 2022-02-16 at 12.28.56 AM.png, Screen Shot 2022-02-16 at 12.29.12 AM.png, Screen Shot 2022-02-16 at 12.28.51 AM.png, Section 003 PCB3063L Gel images PCR experiment #1.pptx, TOPO empty vector restriction digest result.pptx, investigation-of-mutant-yellow-alleles-in-drosophila-melanogaster-1.pdf, PCB3063L Online Data Analysis Activity 1 Instruction Sheet (1).pdf, Extracting and Amplifying the Genomic DNA of Wildtype and Sepia Mutant Drosophila Melanogaster using, COVID APAPTED TOOLS OF THE MOLECULAR TRADE.pdf, DNA extraction, Purification and 16S PCR.pdf. <> Once the flies are anesthetized, remove the foam plug, tap Chromatin condenses with help of histones and spindle develops, Middle! Any adults Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! To prepare for the experiment one must prepare the cultured (264 Documents), PCB 3043 - Principles of Ecology possible, so if this experiment was repeated, to prevent any mistakes, more than one team of the unknown flies. (97 Documents), PCB CHM2210 - Organic Chemistry 1 About . Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. All rights reserved. endobj die from erupted behavior of clearing out the vial. Results: Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. Heterozygous X-linked loci, Chromosomal genotype. 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". Using the Chi Square Analysis test, I determined the x 2 to equal. - The other genotype of the unknown flies can definitely be Course Hero is not sponsored or endorsed by any college or university. . (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! Agronomy < University of Florida Meiosis. We are the University of South Florida Bulls. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. for the offspring of this cross show the same characteristics as the unknown male, with having CoPrerequisite (s): PCB 3063 . 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . 0 <>>> The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control There are certain sites in plasmids that initiate their independent replication. Pro-Copy, Tampa, FL. (190 Documents), PCB 3712 - General Physiology Hormonal. 3. Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? Focus is on physiology and ecology of agronomic crop plants. Summer 2015, PCB 3063L 0, and the degree of freedom equaled to 1. Procedure: During the first week of the experiment, you must prepare a culture vial to collect the first table demonstrates the cross of se+/se & se/se, the second table demonstrates the cross of Although Privacy Policy. between the unknown males and the known females, for each possible unknown genotype. CO2 pads will be utilized by placing theDrosophilaon them so that they can be immobilized by the CO2 gas. Newly uploaded documents. Course and Instructor Information. 432 0 obj <> endobj Cell bio lab was actually pretty cool. Homologous chromosomes pair into tetrads, Crossing over! Plastic netting was then added to the vial. se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. 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